Properties of a coronavirus isolated from a cow with epizootic diarrhea
Identifieur interne : 001715 ( Main/Exploration ); précédent : 001714; suivant : 001716Properties of a coronavirus isolated from a cow with epizootic diarrhea
Auteurs : H. Akashi [Japon] ; Y. Inaba [Japon] ; Y. Miura [Japon] ; S. Tokuhisa [Japon] ; K. Sato [Japon] ; K. Satoda [Japon]Source :
- Veterinary Microbiology [ 0378-1135 ] ; 1980.
English descriptors
- Teeft :
- Adult cattle, American strain, American strain kakegawa, Angeles strain, Bovine, Bovine coronavirus, Bovine enterovirus, Buoyant density, Calf diarrhea, Calf diarrhea coronavirus, Chloroform, Coronavirus, Coronaviruses, Cytopathic changes, Electron microscopy, Epizootic diarrhea, Equal volume, Ether, Hemagglutination inhibition tests, Hemagglutinin activity, Inaba, Infectivity, Iudr, Kakegawa, Maintenance medium, Membrane filter, Neonatal calf diarrhea, Pore size, Publishing company, Reference viruses, Sato, Serum dilution, Takahashi, Trypsin, Virus suspension, Water bath, Water phase.
Abstract
Abstract: A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.
Url:
DOI: 10.1016/0378-1135(80)90025-5
Affiliations:
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Tokuhisa</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Animal Health, Tsukuba, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Sato, K" sort="Sato, K" uniqKey="Sato K" first="K." last="Sato">K. Sato</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Animal Health, Tsukuba, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author><author><name sortKey="Satoda, K" sort="Satoda, K" uniqKey="Satoda K" first="K." last="Satoda">K. Satoda</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Animal Health, Tsukuba, Ibaraki</wicri:regionArea><wicri:noRegion>Ibaraki</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Microbiology</title><title level="j" type="abbrev">VETMIC</title><idno type="ISSN">0378-1135</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">5</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="276">276</biblScope></imprint><idno type="ISSN">0378-1135</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1135</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adult cattle</term><term>American strain</term><term>American strain kakegawa</term><term>Angeles strain</term><term>Bovine</term><term>Bovine coronavirus</term><term>Bovine enterovirus</term><term>Buoyant density</term><term>Calf diarrhea</term><term>Calf diarrhea coronavirus</term><term>Chloroform</term><term>Coronavirus</term><term>Coronaviruses</term><term>Cytopathic changes</term><term>Electron microscopy</term><term>Epizootic diarrhea</term><term>Equal volume</term><term>Ether</term><term>Hemagglutination inhibition tests</term><term>Hemagglutinin activity</term><term>Inaba</term><term>Infectivity</term><term>Iudr</term><term>Kakegawa</term><term>Maintenance medium</term><term>Membrane filter</term><term>Neonatal calf diarrhea</term><term>Pore size</term><term>Publishing company</term><term>Reference viruses</term><term>Sato</term><term>Serum dilution</term><term>Takahashi</term><term>Trypsin</term><term>Virus suspension</term><term>Water bath</term><term>Water phase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A coronavirus (Kakegawa isolate) isolated from a cow with epizootic diarrhea was grown in BEK-1 cells and examined for biophysical and biochemical properties. The Kakegawa isolate was able to replicate in the presence or absence of 5-iodo-2′-deoxy-uridine, indicating that its viral nucleic acid was RNA. It was highly sensitive to ether and chloroform, and moderately sensitive to trypsin and heat. It was, however, readily stabilized by treatment with cation at 50°C for 1 h. Its infectivity was slightly reduced at pH 3.0. The virus passed through a membrane filter of 200 nm pore size, but not through one of 100 nm pore size. The buoyant density of the virus was determined in a sucrose density gradient. The peak of infectivity and hemagglutinin activity was found at a density of 1.182. Neutralization and hemagglutination inhibition tests showed a close serological relationship between the Kakegawa isolate and the American strain of calf diarrhea coronavirus.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Akashi, H" sort="Akashi, H" uniqKey="Akashi H" first="H." last="Akashi">H. Akashi</name></noRegion><name sortKey="Inaba, Y" sort="Inaba, Y" uniqKey="Inaba Y" first="Y." last="Inaba">Y. Inaba</name><name sortKey="Miura, Y" sort="Miura, Y" uniqKey="Miura Y" first="Y." last="Miura">Y. Miura</name><name sortKey="Sato, K" sort="Sato, K" uniqKey="Sato K" first="K." last="Sato">K. Sato</name><name sortKey="Satoda, K" sort="Satoda, K" uniqKey="Satoda K" first="K." last="Satoda">K. Satoda</name><name sortKey="Tokuhisa, S" sort="Tokuhisa, S" uniqKey="Tokuhisa S" first="S." last="Tokuhisa">S. Tokuhisa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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